Journal: Frontiers in Cellular Neuroscience

Article Title: (Ba,Ca)(Ti,Sn)O 3 -based piezoelectric ceramics promotes neuroprotection by regulating microglial IL-6/JAK2/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1793008

Figure Lengend Snippet: BCTS-CM attenuates activation of the IL-6/JAK2/STAT3 pathway in PC12 cells. (A) Western blot results of IL-6/JAK2/STAT3 signaling pathway. (B,C) Statistical analysis of P-JAK2/JAK2 and P-STAT3/STAT3 expression. Data are expressed as mean ± SEM, n = 5 vs. Con group, a P ≤ 0.01 vs. LPS group, b P ≤ 0.05 vs. BCTS-CM group, c P ≤ 0.05.

Article Snippet: JSM-5900 Scanning Electron Microscope (Japan), Xpert-PRO X-ray Diffractometer (Netherlands), Water Contact Angle Goniometer JC2000D1 (Shanghai Zhongchen Digital Technology Equipment Co., Ltd., China), Quasi-static d 33 Meter ZJ-3AN (Institute of Acoustics, Chinese Academy of Sciences, China), Ultrasound Stimulator (GV-CSI1.0) (Green Valley BrainTech (ShenZhen) Medical Technology Co., Ltd. (China), BV2, PC12 (Wuhan Pricella Biotechnology Co., Ltd., China), Rabbit Anti-iNOS (Thermo Fisher Scientific, Inc.), Rabbit Anti-Iba-1, Arg-1, NeuN, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488), Goat Anti-Mouse IgG H&L (Alexa Fluor 594) (Abcam, UK), Rabbit Anti-CD86, CD206, β-actin, HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Wuhan Proteintech Biotechnology Co., Ltd., China), Rabbit Anti-IL-6, TNF-α, IL-4, IL-10, TGF-β1 (ImmunoWay, United States), Rabbit Anti-IL-1β, JAK2, P-JAK2, STAT3, P-STAT3, BAX, Bcl-2, Cleaved Caspase-3 (Shenyang Wanlei Biotechnology Co., Ltd., China), BCA Protein Assay Kit, CCK8 Cell Viability Assay Kit, LDH Cytotoxicity Assay Kit, SOD Activity Assay Kit, CAT Activity Assay Kit, GSH-Px Activity Assay Kit, MDA Assay Kit, Dapi (Beijing Solarbio Science & Technology Co., Ltd., China), LPS (Sigma, Germany), IL-6, AG490 (MCE, Germany), Laser Confocal Microscope (Olympus Corporation, Japan), SDS-PAGE Electrophoresis System (BIO-RAD, USA).

Techniques: Activation Assay, Western Blot, Expressing

Journal: Frontiers in Cellular Neuroscience

Article Title: (Ba,Ca)(Ti,Sn)O 3 -based piezoelectric ceramics promotes neuroprotection by regulating microglial IL-6/JAK2/STAT3 signaling pathway

doi: 10.3389/fncel.2026.1793008

Figure Lengend Snippet: BCTS attenuates the expression of the IL-6/JAK2/STAT3 signaling pathway . (A) Western blot results of IL-6/JAK2/STAT3 signaling pathway. (B–D) Statistical analysis of IL-6, P-JAK2/JAK2 and P-STAT3/STAT3 expression. Data are expressed as mean ± SEM, n = 5 vs. Sham group, a P < 0.05, b P < 0.01 vs. SCI group, c P < 0.05.

Article Snippet: JSM-5900 Scanning Electron Microscope (Japan), Xpert-PRO X-ray Diffractometer (Netherlands), Water Contact Angle Goniometer JC2000D1 (Shanghai Zhongchen Digital Technology Equipment Co., Ltd., China), Quasi-static d 33 Meter ZJ-3AN (Institute of Acoustics, Chinese Academy of Sciences, China), Ultrasound Stimulator (GV-CSI1.0) (Green Valley BrainTech (ShenZhen) Medical Technology Co., Ltd. (China), BV2, PC12 (Wuhan Pricella Biotechnology Co., Ltd., China), Rabbit Anti-iNOS (Thermo Fisher Scientific, Inc.), Rabbit Anti-Iba-1, Arg-1, NeuN, Goat Anti-Rabbit IgG H&L (Alexa Fluor 488), Goat Anti-Mouse IgG H&L (Alexa Fluor 594) (Abcam, UK), Rabbit Anti-CD86, CD206, β-actin, HRP-conjugated Goat Anti-Rabbit IgG (H + L) (Wuhan Proteintech Biotechnology Co., Ltd., China), Rabbit Anti-IL-6, TNF-α, IL-4, IL-10, TGF-β1 (ImmunoWay, United States), Rabbit Anti-IL-1β, JAK2, P-JAK2, STAT3, P-STAT3, BAX, Bcl-2, Cleaved Caspase-3 (Shenyang Wanlei Biotechnology Co., Ltd., China), BCA Protein Assay Kit, CCK8 Cell Viability Assay Kit, LDH Cytotoxicity Assay Kit, SOD Activity Assay Kit, CAT Activity Assay Kit, GSH-Px Activity Assay Kit, MDA Assay Kit, Dapi (Beijing Solarbio Science & Technology Co., Ltd., China), LPS (Sigma, Germany), IL-6, AG490 (MCE, Germany), Laser Confocal Microscope (Olympus Corporation, Japan), SDS-PAGE Electrophoresis System (BIO-RAD, USA).

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: LncTUG1 promotes hepatocellular carcinoma immune evasion via upregulating PD-L1 expression

doi: 10.1038/s41598-023-42948-8

Figure Lengend Snippet: TUG1 affects the JAK2/ STAT3 pathway to regulate PD-L1. ( A ): The relative mRNA expression of JAK2 was analyzed in shTUG1 HCC cells. ( B ): The relative mRNA expression of STAT3 was analyzed in shTUG1 HCC cells. ( C ): The protein levels of pJAK2, JAK2, pSTAT3, STAT3, PD-L1 were analyzed in shTUG1 HCC cells by western blotting. ( D ) pJAK2, JAK2,and PDL1 protein levels are assessed by immunoblotting in HCC-LM3 cell was transfected with TUG1 silenced (shTUG1) and/or JAK2 overexpressed (JAK2-OE). ( E ) pSTAT3,STAT3,and PDL1 protein levels are assessed by immunoblotting in HCC-LM3 cell was transfected with TUG1 silenced (shTUG1) and/or STAT3 overexpressed (STAT3-OE).

Article Snippet: Protein detection was performed by using the following primary antibodies: anti-PD-L1 (ab205921, Abcam), anti-GAPDH (ab8245, Abcam), anti-JAK2 (#3230, Cell Signaling Technology), anti-pJAK2 Tyr1007/1008 (#3771,Cell Signaling Technology),anti-STAT3 (#9139, Cell Signaling Technology), anti-pSTAT3 Tyr705 (#9145, Cell Signaling Technology).

Techniques: Expressing, Western Blot, Transfection

Journal: Chemistry & biology

Article Title: Selecting agonists from single cells infected with combinatorial antibody libraries.

doi: 10.1016/j.chembiol.2013.04.012

Figure Lengend Snippet: Figure 5. Signal Transduction by Agonist Antibodies JAK2 was purified from cell lysates using affinity to anti-JAK2 agarose and its phosphorylation was detected with western blotting using anti- phosphotyrosine antibodies. Phosphorylation of STAT3, STAT5, Akt, and MAPK induced by agonist antibodies or rhTPO stimulation was detected with direct western blotting of cell lysates. See also Figure S6.

Article Snippet: The immunoprecipitates were analyzed with western blotting using anti-phospho-JAK2 (Tyr1007/1008) (C80C3) antibody (Cell Signaling Technology, catalog no. 3776) and anti-total JAK2 antibody (C-14) (Santa Cruz Biotechnology, catalog no. sc-34479).

Techniques: Transduction, Phospho-proteomics, Western Blot

Journal: Life

Article Title: Anticancer Effects of Weizmannia coagulans MZY531 Postbiotics in CT26 Colorectal Tumor-Bearing Mice by Regulating Apoptosis and Autophagy

doi: 10.3390/life14101334

Figure Lengend Snippet: Western blotting diagram of the effect of W. coagulans MZY531 postbiotics on the expression of apoptosis signaling pathway proteins. ( A ) Bax, Bcl2, and caspase-3 protein expressions in tumor tissue. ( B – D ) Bax, Bcl2, and caspase-3 protein expression gray value. ( E ) Phosphorylation of JAK2 and STAT3 and the expression levels of unphosphorylated JAK2 and STAT3 proteins in tumor tissue. ( F , G ) P-JAK2/JAK2 and P-STAT3/STAT3 protein expression gray value. Statistical analysis was conducted using ANOVA, with subsequent post hoc testing performed via Duncan’s multiple comparison test. Means with different superscript letters (a, b, c, and d) are statistically significantly different from each other ( p < 0.05), with ‘a’ representing the highest value and ‘d’ representing the lowest.

Article Snippet: Following blocking with 5% bovine serum albumin, the membranes were incubated with the appropriate antibodies: β-actin, p-JAK2, STAT3, Bcl-2 and Bax (GeneTex, San Antonio, TX, USA), JAK2, p-STAT3, PI3K, AKT, mTOR and p-mTOR (Bioss, Beijing, China), caspase-3 (Abcam, Cambridge, MA, USA), TGF-β, and SMAD4 (Proteintech, Wuhan, China).

Techniques: Western Blot, Expressing, Phospho-proteomics, Comparison

Journal: Scientific Reports

Article Title: Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2–STAT3 pathway

doi: 10.1038/s41598-021-91008-6

Figure Lengend Snippet: Daphnetin inhibits activation of the JAK2–STAT3 pathway in the lungs of mice with PALI. ( A ) Western blot analysis of p-JAK2, JAK, p-STAT3 and STAT3 expression in lung of each group, Bar graph showed the ratio of p-JAK2: total JAK2 protein ( B ). p-STAT3: total STAT3 protein ( C ). Full-length blots are presented in Supplementary Figure . The data are presented as the means ± SD. The histograms were created using GraphPad 5 software(GraphPad Prism 5.01 http://www.xdowns.com/soft/xdowns2009.asp?softid=49668&downid=60&id=52443 ). The quantitative analysis of blots was used by ImageJ software(Image J 2 system software https://imagej.net/Downloads ). Full-length blots are presented in Supplementary Figure . CON group, l-arginine vehicle + daphnetin vehicle; SAP group, l-arginine + daphnetin vehicle; DAP group, l -arginine vehicle + daphnetin; SAP + DAP group, l -arginine + daphnetin. n = 3 /group, data are representative of three independent experiments. ** P < 0.01 versus CON group; ## P < 0.01 versus SAP group, n.s. P > 0.05 versus CON group.

Article Snippet: Primary antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), JAK2, and STAT3 were purchased from Proteintech (10494-1-AP, 17670-1-AP, 10253–2-AP), and primary antibodies against the phosphorylated forms of JAK2 and STAT3 (p-JAK2 and p-STAT3) were obtained from Cell Signaling Technology (4406T, 9145T).

Techniques: Activation Assay, Western Blot, Expressing, Software

Journal: Scientific Reports

Article Title: Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2–STAT3 pathway

doi: 10.1038/s41598-021-91008-6

Figure Lengend Snippet: Daphnetin inhibits activation of the JAK2–STAT3 pathway in the lungs of mice with PALI. Immunohistochemical staining of p-JAK2 ( A ) and p-STAT3 ( B ) in lung of each group. The magnification of each histological images were × 200. Quantitative analysis of p-JAK2 ( C ) and p-STAT3 ( D ). The data are presented as the means ± SD. The histograms were created using GraphPad 5 software (GraphPad Prism 5.01 http://www.xdowns.com/soft/xdowns2009.asp?softid=49668&downid=60&id=52443 ). The quantitative analysis of immunostaining was used by ImageJ software(Image J 2 system software https://imagej.net/Downloads ). CON group, l-arginine vehicle + daphnetin vehicle; SAP group, l-arginine + daphnetin vehicle; DAP group, l-arginine vehicle + daphnetin; SAP + DAP group, l-arginine + daphnetin. ** P < 0.01 versus CON group; ## P < 0.01 versus SAP group, n.s. P > 0.05 versus CON group. Scale bars: 50 μm.

Article Snippet: Primary antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), JAK2, and STAT3 were purchased from Proteintech (10494-1-AP, 17670-1-AP, 10253–2-AP), and primary antibodies against the phosphorylated forms of JAK2 and STAT3 (p-JAK2 and p-STAT3) were obtained from Cell Signaling Technology (4406T, 9145T).

Techniques: Activation Assay, Immunohistochemical staining, Staining, Software, Immunostaining